Ecotropic Receptor Booster
Ecotropic receptor booster technologies are used to temporarily elevate the density of viral receptor proteins on the surface of any target cell that you wish to infect. The technology allows you to efficiently transduce cells that are resistant to viral infection due to low receptor density. The Ecotropic Receptor Booster, for example, allows you to efficiently transduce human cells with ecotropic retrovirus or lentivirus that otherwise can only infect rodent cells.
Receptor booster technologies are used to temporarily elevate the density of viral receptor proteins on the surface of any target cell that you wish to infect. The technology allows you to efficiently transduce cells that are resistant to viral infection due to low receptor density. The Ecotropic Receptor Booster allows you to efficiently transduce human cells with ecotropic retrovirus or lentivirus that otherwise can only infect rodent cells.
How does it work?
Each receptor booster consists of a vial of concentrated exosome-like vesicles that are coated with the mCAT-1 receptor protein. When the booster vesicles are applied to your cells (10–20 µl per well of a 6-well plate for ~2 hours), they fuse with the plasma membrane, resulting in an increased level of the receptor on the cell surface. Your target cells can then be more efficiently transduced with ecotropic retrovirus.
We recommend the following products for producing ecotropic or amphotropic virus:
-
Ecotropic lentivirus: Lenti-X Packaging Single Shots (Ecotropic)
- Ecotropic retrovirus: Retro-X Universal Packaging System
Overview
- Increase efficiency of transduction by temporarily increasing the concentration of viral receptor proteins on your target cells
- Simply preincubate your target cells with these concentrated exosome-like vesicles for 2 hours prior to infection with virus
- Infect human cells with ecotropic retrovirus or ecotropic lentivirus
- The Ecotropic Receptor Booster (Cat. # 631471) delivers the mCAT-1 receptor to target cells
More Information
Applications
- Increased transduction efficiency of hard-to-infect cell types
- Transduce human cells with ecotropic retrovirus or lentivirus
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
Recombinant retrovirus frequently asked questions
What is cloning capacity? What is an infectious unit? How do I concentrate my virus? Find answers to common questions related to gene delivery using recombinant retrovirus.
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